Skip to main content
. 2021 Jul 12;9(7):806. doi: 10.3390/biomedicines9070806

Figure 2.

Figure 2

rSur-FLIPr is efficiently captured by dendritic cells. Groups of C57BL/6 mice were injected with Alexa 647-labeled rSur or rSur-FLIPr in the hind foot pads (100 μg/foot pad). Mice injected with PBS were used as controls. Draining lymph nodes were harvested at 24 and 40 h after injection. (a) Gating strategy of the DC population in mouse lymph nodes. Single cells were gated by FSC-W/SSC-A. Dead cells were removed from the analysis using LIVE/DEAD® fixable dead cell stains. B cells, T cells, NK cells, and neutrophils were excluded from the analysis by staining with CD19, CD3e, NK1.1, and Ly6G (1A8) antibodies. CD11c+MHCII+ cells were further analyzed for CD11c and labeled antigen. (b) MHCII+CD11c+ cells were analyzed for the expression of labeled antigens. The results were pooled from three independent experiments and expressed as the mean ± SE of the mean (n = 8). The statistical significance was determined using the Kruskal–Wallis test with Dunn’s multiple comparison test. ** p < 0.01.