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Gene expression analyses of Col4a3, Nos3, Pdgf, Fgfb, Vegf, Sphk1, Hif1a, and Eng. The RT-qPCR assays were performed in newly extracted ad-MFVs and sprouting ad-MFVs at day 1 and day 8 in 3D collagen type-I hydrogel. The gene expression levels were analysed using the 2-ΔΔCt method using Gapdh as the reference gene. The data represent the mean ± SD of three independent culture experiments. Bars with an asterisk are significantly different at p < 0.05.
