Oral cancer biomarker proteins detected in 1% calf serum in PBS by the amperometric microfluidic array after incubation of Ab2-MB-HRP-analytes in detection chamber, then injecting mixture of H2O2 and HQ: (A) duplicate reproducible peaks in simultaneous measurements of a mixture of 10 fg mL−1 IL-6, 15 fg mL−1 IL-8, 25 fg mL−1 VEGF, and 60 fg mL−1 VEGF-C, (B) VEGF peaks in mixtures of all biomarker proteins. (C–F) Immunoarray calibrations of background corrected peak currents for IL-6 (C), IL-8 (D), VEGF (E), VEGF-C (F). Error bars are standard deviations for n = 6. Reprinted from [26]. Copyright 2012 American Chemical Society.