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Protein concentrations found using online cell lysis of single cell or cell-free samples filtered and washed to remove cell culture media for cancer cell cultures (A) HN12, (B) HN13, (C) HN30, and (D) CAL27 (n = 6). Number of cells was estimated using the β-Tub concentration per single cell. Zero cell or single cell samples are marked on the graphs for DSG3 and VEGF-A. Adapted from [43], reprinted with permission. Copyright Elsevier, 2021.
