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. 2021 Jul 9;9(7):797. doi: 10.3390/biomedicines9070797

Figure 2.

Figure 2

TGF-β and BMP-4 have a synergistic effect on human ASC differentiation. hASCs were stimulated either with TGF-β, BMP-4 or in combination (DM) for up to 7 days and αSMA (A), caldesmon (B) and SM22α (C) expression were evaluated by Western blot and fluorescence microscopy (D). Scale bar 100 µm. Top are representative immunoblots for αSMA, caldesmon and SM22α expression. Corresponding line graph demonstrating the time-course (0 to 7 days) effect of TGF-β, BMP-4 or DM on αSMA, caldesmon and SM22α expression. Results are mean ± SEM of 5 experiments. * p < 0.05 vs. day 0 of stimulation with DM; ** p < 0.05 vs. day 0 of stimulation with TGF-β; # p < 0.05 vs. day 0 of stimulation with BMP-4.