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. 2021 Jul 9;9(7):797. doi: 10.3390/biomedicines9070797

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Combination of IGFBP7 and BMP-4 but not IGFBP7 alone induced expression of molecular markers of SMC. Human ASCs were stimulated with differentiation media containing TGF-β and BMP-4 (DM), IGFBP7 or combination of IGFBP7 and BMP-4 for up to 7 days. Top are representative immunoblots for αSMA (A), caldesmon (B) and SM22α (C) expression. Corresponding line graph demonstrating the time-course (0 to 7 days) effect of DM, IGFBP7 or IGFBP7 and BMP-4 on αSMA, caldesmon and SM22α expression. (D) Western blot analysis was further confirmed by fluorescence microscopy to detect αSMA, caldesmon and SM22α expression at day 0, 4 and 7 following stimulation with DM, IGFBP7 or IGFBP7 and BMP-4. Scale bar 100 µm. Results are mean ± SEM of 5 experiments. * p < 0.05 vs. day 0 of stimulation with DM; ** p < 0.05 vs. day 0 of stimulation with or IGFBP7 and BMP-4.