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. 2021 Jul 9;9(7):797. doi: 10.3390/biomedicines9070797

Figure 5.

Figure 5

TGF-β- and BMP-4-induced differentiation of human ASCs is stable for up to 21 days. (A) Top are representative immunoblots for αSMA, caldesmon and SM22α expression. Corresponding bar graph demonstrating the stability of differentiation when cells were differentiated for 7 days and then either maintained in differentiation media containing TGF-β and BMP-4 or transferred to DMEM supplemented with FBS for indicated times. (B) Fluorescence microscopy was also used to detect αSMA in hASsC differentiated for 7 days and either kept in DM or switched to DMEM supplemented with FBS for indicated times. Scale bar 100 µm. (C) Contractile activity of non-differentiated hASCs and hASCs differentiated for 7 days and then either maintained in differentiation media containing TGF-β and BMP-4 or transferred to DMEM supplemented with FBS for indicated times. The collagen gel matrices were cultured in the serum-free medium for up to 6 h and gel contraction was photographed at the indicated time points using a digital camera. Corresponding line graph demonstrating the time-course (0 to 6 h) effect of 60 mM of KCl on the area of the gel lattices relative to its original size. Results are mean ± SEM of 5 experiments. * p < 0.05 vs. non-differentiated ASC.