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. 2021 Jul 9;9(7):797. doi: 10.3390/biomedicines9070797

Figure 7.

Figure 7

PET structures do not affect the differentiation of human ASCs into SMC mediated by TGF-β and BMP-4. Long-term stability of differentiation was evaluated on hASCs grown in PET surface. Cells were differentiated for 7 days and then either maintained in differentiation media containing TGF-β and BMP-4 or transferred to DMEM supplemented with FBS for indicated times. (A) Top are representative immunoblots for αSMA, SM22α and caldesmon expression. Corresponding bar graph demonstrate protein expression of αSMA and caldesmon evaluated by Western blot. (B) Fluorescence microscopy was also used to detect αSMA, SM22α and caldesmon expression in ASCs grown in PET surface at indicated time points. Scale bar 100 µm. Results are mean ± SEM of 5 experiments. * p < 0.05 vs. non-differentiated ASC.