NNMT inhibition protects against alcoholic fatty liver development. Ten-week old male C57BL/6N mice were retro-orbitally injected with either Ad-GFP-U6-m-NNMT-shRNA or corresponding control vector, followed by the NIAAA model regimen. (A) Hepatic NNMT protein abundance; (B) Plasma TAG levels, (C) Plasma total cholesterol levels; (D) Plasma glucose levels; (E) The liver TAG levels. (F) H&E staining of liver tissues. Ten-week old male C57BL/6N mice were injected (ip) with either PBS or JBSNF-00008, a chemical inhibitor of NNMT, twice a day in the NIAAA model regimen. (G) The liver TAG levels; (H) H&E staining of liver tissues. AML12 cells were treated with JBSNF-00008 (10 μM), a chemical inhibitor of NNMT, for indicated time durations. (I) Intracellular TAG levels were measured. Data are expressed as mean ± SEM (n = 5–8). Differences between groups were determined using one-way ANOVA analysis (*p < 0.05 versus control vector/PF mice).