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. 2021 Jul 23;16(7):e0255257. doi: 10.1371/journal.pone.0255257

Fig 8. Effect of duration and staining on DNA quality and variant calling in 21 smear samples.

Fig 8

(A) The dsDNA/total DNA ratio was plotted chronologically starting from smear preparation. (B) The dsDNA/total DNA ratio was compared between MGG-stained and unstained smear samples. (C) Bar plot of the amount of filtered and remaining variants. Filtered variants are shown separately by the filtering methods used (FilterMutectCall: orange; VAF 2.5 or less: blue) in the upper panel. Variants after filtering are shown in the lower panel. The quantity of variants removed by FilterMutectCalls (D) and the quantity removed at VAF 2.5 or a lower level of detection (E) were compared between the MGG-stained and unstained smear samples.