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. 2021 Jul 13;17(7):e1009311. doi: 10.1371/journal.ppat.1009311

Fig 9. Stable Suppression of Sp1 inhibits T. denticola-stimulated upregulation of MMP 2, 11, 14, 17 and 28 in hPDL cells.

Fig 9

A) RT-qPCR validation of stable gene suppression using shRNA vectors targeted against Sp1 in healthy hPDL cells. Cells transduced with scrambled shRNA vectors were used as a control. Statistical significance was determined using an unpaired t-test. Bars represent ± SEM of mean values (n = 3 clones). **p < .01 versus control. B-F) RT-qPCR for MMP-2, MMP-11, MMP-14, MMP-17 and MMP-28 mRNA expression of scrambled shRNA control and Sp1 shRNA hPDL cells challenged or stimulated with purified dentilisin at a concentration of 1 μg/mL or Td-WT at an MOI of 50 for 2-hours in alpha-MEM media supplemented with 10% FBS and no antibiotics followed by a 22-hour incubation in alpha-MEM media supplemented with 10% FBS, 1% Pen/Strep and 1% Amphotericin B. The expression of each gene was normalized to that of GAPDH. Statistical significance was determined using a Two-Way ANOVA followed by post-hoc Tukey’s multiple comparisons. Bars represent mean ± SEM (n = 3). #p < .05 versus scramble control group. ##p < .01 versus scramble control group. ###p < .001 versus scramble control group. *p < .05 versus Sp1 shRNA equivalent group. **p < .01 versus Sp1 shRNA equivalent group. ***p < .001 versus Sp1 shRNA equivalent group.