Low amphetamine doses in adolescence do not alter mesocortical dopamine connectivity in adulthood. A, Timeline of treatment and experimental procedures. B, Schematic representation of the regions of interest in the medial prefrontal cortex outlined according to the Mouse Brain Atlas (Paxinos and Franklin, 2008). The cingulate (Cg1), prelimbic (PrL), and infralimbic (IL) subregions of the medial prefrontal cortex were analyzed. C, Volume, D, total number, and E, density of the TH-positive fiber innervation to the inner layers of the medial prefrontal cortex. Mice treated with 0.5 mg/kg of AMPH in adolescence do not show significant differences to their saline counterparts. All data are shown as mean ± SEM (n = 4-6 per group)