Table 1.

Primer sets for real-time RT-PCR: plasma membrane Ca²⁺ ATPase (PMCA) and odontoblast marker proteins.

Name	5′-Sequence-3′	GenBank Number
β-actin	Forward TGGCACCCAGCACAATGAA Reverse CTAAGTCATAGTCCGCCTAGAAGCA	NM_001101.5
PMCA1	Forward ACCATATGCTAGAATGCCCACCTC Reverse CTGGTGAAATCTGGGCCCTAAC	NM_001001323.2
PMCA2	Forward AGAGCTTCCGCATGTACAGCAA Reverse CAAGCCATGGGCTCAATCAC	NM_001001331.4
PMCA3	Forward CGTAACGTCTATGACAGCATCTCCA Reverse TCCATGATCAAGTTCACCCACAA	NM_001001344.2
PMCA4	Forward TGGCATGGTTAAATCTGAATGG Reverse CTGCTTCAATTGTAAGGCAAAGG	NM_001001396.2
DMP-1	Forward TCCAGTCTCACAGCAGCTCA Reverse TCTCCGTGGAGTTGCTATCTTC	NM_004407.4
DSPP	Forward TGATAGCAGTGACAGCACATCTGAC Reverse GTTGTTACCGTTACCAGACTTGCTC	NM_014208.3

The conditions for real-time RT-PCR were as follows: 1 cycle at 42 °C for 5 min, followed by 1 cycle at 95 °C for 10 s, 40 cycles at 95 °C for 5 s, and then 60 °C for 30 s. The conditions for dissociation curve analysis were as follows: 1 cycle at 95 °C for 15 s, 1 cycle at 60 °C for 30 s, and 1 cycle at 95 °C for 15 s.