Figure 10.

Comparison of cryo-EM structure (PDB id: 6IYC) with bound APP-C83 (purple helix and β-thread) with structures of Aβ peptides from exemplary MD simulations (helix in salmon). Olive and brown balls indicate the positions of the same residue to show possible rotations and movements of Aβ₄₃/Aβ₄₅ (olive) compared with APP-C83 (brown). (a) Superimposition of PS-1 with APP-C83 substrate and PS-1 with docked Aβ₄₃ substrate after MD simulation of the γ-secretase complex in 10% cholesterol membrane; (b) the same in 50% cholesterol membrane; (c) superimposition of PS-1 with APP-C83 substrate and PS-1 with docked Aβ₄₅ substrate after MD simulation of the γ-secretase complex in 10% cholesterol membrane; (d) the same in 50% cholesterol membrane.