Figure 2.

Overview of the protocol for the generation of a hepatic lobule array. Kang et al. fabricated a preset cartridge and injected hepatocyte-laden bioinks, ECs-laden bioinks, and cell-free bioinks into it to imbed HepG2/C3A and ECs. Then, they removed the alginate bioink to form a lumen. After incubation at 37 °C, ECs covered the outside of the construct, forming a lumen in which ECs were lined and interconnected between the exterior and the lumen.