Figure 5.

fMLP quantification by LC-MS/MS for the protein degradation test against MRSA 700699. (A) Control (without any antimicrobial agents), (B) LZD, (C) SeNP, (D) Combination (LZD + SeNP), (E) calculated amount of fMLP, and (F) normalized amount of fMLP to CFU. A protein degradation test was performed with only MRSA 700699, which showed the greatest synergistic effect in Figure 4. The CFU values used in (B) are shown in Figure 4B. The control was a bacterial solution without any antimicrobial agents. The concentrations of LZD and SeNP were the same as those used in Figure 4. In (F), as expected, the control group showed the lowest protein degradation value, and the combi group showed more protein degradation per bacterium than the single administration groups. Therefore, it seems that LZD and SeNPs showed synergistic effects through protein degradation.