Figure 5.

(A) Concentration-dependent effect of 1a, 1b and 2 (1 and 2 µM) on the actin cytoskeleton of HT-29 colon adenocarcinoma cells. An equivalent amount of solvent (DMSO) served as negative control. Images are representative of at least three independent experiments. Magnification 630× (n = 3 per group). (B) Substance 1a (1 µM) causes blebbing (I., bright field), nuclei fragmentation (II., DAPI staining) and different types of actin reorganization (III. and IV., phalloidin staining) such as stress fiber formation, actin clustering, cell rounding and formation of apoptopodia-like structures in HT-29 cells (n = 3 per group).