Table 1.
The propensity for glycosylation of monomeric DBD of p5396-291 taken from the crystal structure of a dimer of dimers of DBD bound to two decamers of DNA (PDB ID: 2AHI, resolution 1.8 Å, [29]). The first column is the sequence from residues i − 2 to i + 2 (residue i is marked with *); the N-terminus sequence is from i to i + 2 or i − 1 to i + 2. The second column is the identity of the chain in the pdb file. The third column is the residue number (i) of the Thr or Ser residue. The fourth column is the GLYCAM score of the particular Ser or Thr in monomeric DBD while the fifth column lists the glycosylation propensity of the residue, i.e., positive (+), zero (0), and negative (−), corresponding to high, low, and zero propensity for glycosylation. The sixth column is the difference between the GLYCAM score of the monomeric wild type DBD and the monomeric Y163C mutant DBD. The seventh column is the difference between the GLYCAM score of the monomeric wild type DBD and the monomeric Q167K mutant DBD.
| Residue Name | Chain | Residue Number | GlyCAM Score for Wild Type Monomeric DBD | Glycosylation Status | Difference Compared to Wild Type Monomeric DBD | |
|---|---|---|---|---|---|---|
| Y163C | Q167K | |||||
| S*VP | A | 96 | 99.8 | + | 0 | 0 |
| VPS*QK | A | 99 | 57.7 | + | 0 | 0 |
| QKT*YQ | A | 102 | 51.9 | + | 0 | 0 |
| QGS*YG | A | 106 | 89.2 | + | 0 | 0 |
| LHS*GT | A | 116 | 31.5 | 0 | 0 | 0 |
| SGT*AK | A | 118 | 19.4 | − | 0 | 0 |
| AKS*VT | A | 121 | 56.3 | + | 0 | 0 |
| SVT*CT | A | 123 | 56.8 | + | 0 | 0 |
| TCT*YS | A | 125 | 1.3 | − | 0 | 0 |
| TYS*PA | A | 127 | 0 | − | 0 | 0 |
| AKT*CP | A | 140 | 41.6 | + | 0 | 0 |
| VDS*TP | A | 149 | 69.3 | + | 0 | 0 |
| DST*PP | A | 150 | 63.2 | + | 0 | 0 |
| PGT*RV | A | 155 | 1.3 | − | 0 | 0 |
| KQS*QH | A | 166 | 91.3 | + | 0 | 6.9 |
| HMT*EV | A | 170 | 83.9 | + | 0 | 0 |
| RCS*DS | A | 183 | 111.7 | + | 0 | 0 |
| SDS*DG | A | 185 | 68.3 | + | 0 | 0 |
| RNT*FR | A | 211 | 37.2 | 0 | 0 | 0 |
| RHS*VV | A | 215 | 5.2 | − | 0 | 0 |
| VGS*DC | A | 227 | 41 | + | 0 | 0 |
| DCT*TI | A | 230 | 17.5 | − | 0 | 0 |
| CTT*IH | A | 231 | 35.5 | 0 | 0 | 0 |
| CNS*SC | A | 240 | 13.4 | − | 0 | 0 |
| NSS*CM | A | 241 | 62.2 | + | 0 | 0 |
| ILT*II | A | 253 | 0.7 | − | 0 | 0 |
| IIT*LE | A | 256 | 14.5 | − | 0 | 0 |
| EDS*SG | A | 260 | 70.4 | + | 0 | 0 |
| DSS*GN | A | 261 | 81.2 | + | 0 | 0 |
| RNS*FE | A | 269 | 14.7 | − | 0 | 0 |
| RRT*EE | A | 284 | 70.8 | + | 0 | 0 |