Figure 1.

Illustration of a Layer V YFP-H positive neuron within the motor cortex. Three different regions were included for spine quantification (red boxes). All apical tuft dendrites were quantified in layer I, identified by the clearly visible meninges membrane. Apical dendrites within cortical layer II/III were identified through location of the cortical layers and only the main apical dendrite included in quantification. Basal dendrites in layer V were identified as directly connected to a YFP-H pyramidal cell soma. Dendritic spines on the entire visible dendritic tree were counted from a single somatic branch point. Scale bars 50 µM.