Figure 2.

TDP-43 localization within the motor cortex. (A). Example of WT Layer V neurons within the motor cortex that are positive for YFP (green) and DAPI (blue), with no human TDP-43 (red) labelling present. (B). TDP-43^WT Layer V neurons within the motor cortex were positive for YFP (green), human TDP-43 (red) and DAPI (blue), with human TDP-43 labelling nuclear (arrows) (merged). (C). TDP-43^ΔNLS Layer V neurons within the motor cortex were positive for YFP (green), human TDP-43 (red) and DAPI (blue), with human TDP-43 labelling excluded from the nucleus (arrowheads) (merged). (D). Three-dimensional representation of a YFP neuron (green) with TDP-43 (red) wrapping around the DAPI nucleus (blue). Scale bar, A-C = 50 µm, D = 20 µm.