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Binding of recombinant HSP47 to collagen toolkits in coated 96-well plates was measured as described in Material and Methods. (a) shows binding to toolkit II with collagens II and III as positive controls as indicated, and (b) to toolkit III, with GFOGER as positive control. BSA and GPP10 served as negative control surface coatings. Data are the means + S.E. of three independent experiments.
