Table 1.

Identification of HSP47-binding THPs from the toolkits II and III. GPP represents the start or finish of the [GPP]₅ host peptide, while O represents hydroxyproline (Hyp). The GXR region that is predicted to play a key role in HSP47 binding is colored red. For the sequences that contain more than one GXR motif, we highlighted the most plausible one according to our energy prediction results (Figure S4c). The integrin-binding THPs with a GXOGER sequence flanked by [GPP]₅ at both ends, where X (colored blue in the table) represents F, A, M, L, or R, were used as controls.

Peptide Name	Sequence	Mean A450
Toolkit II
14	---------------------------------------GPPGPRGPOGPQGATGPLGPKGQTGEOGIA GPP	>2
20	------GPPGANGDOGROGEOGLOGARGLTGROGDAGPP	>2
13	-GPPGAKGSAGAOGIAGAOGFOGPRGPOGPQGPP	1.5–2
26	---------------------------------------GPPGERGEQGAOGPSGFQGLOGPOGPOGEGGPP	1.5–2
11	--------------------GPPGARGPEGAQGPRGEOGTOGSOGPAGASGPP	1–1.5
10	GPPGGOGFOGAOGAKGEAGPTGARGPEGAQGPP	0.5–1
17	- GPPGKRGARGEOGGVGPIGPOGERGAOGNRGPP	0.5–1
24	-------------GPPGKAGEKGLOGAOGLRGLOGKDGETGAAGPP	0.5–1
39	---------------------------------------GPPGARGAQGPOGATGFOGAAGRVGPOGSNGPP	0.5–1
Toolkit III
5	---------------------------------------GPPGER GLOGPOGIKGPAGIOGFOGMKGHRGPP	1.5–2
30	--------------------------------GPPGAOGLRGGAGPOGPEGGKGAAGPOGPOGPP	0.5–1
14	--------------------GPPGIOGAOGLMGARGPOGPAGANGAOGLRGPP	0.5–1
Integrin Peptide
1	---------------------------------GPPGFOGERGPP	>2
2	--------------------------------GPPGMOGERGPP	1.5–2
3	---------------------------------GPPGAOGERGPP	1.5–2
4	---------------------------------GPPGLOGERGPP	1.5–2
5	---------------------------------GPPGROGERGPP	0–0.5