Figure 3.

Role of interhelical H-bonds in full length DesK. B. subtiliss. ∆desk cells expressing full length DesK and variants with the indicated mutations were grown at 25 °C and 37 °C, as described in Figure 1C. β-galactosidase activity was measured and expressed in Miller Units (MU). Error bars include the standard deviation from at least three independent experiments. There is not significant difference among activities labeled with the same letter. There is significant difference among activities labeled with different letters. In the bottom panel, TMSs are represented as cylinders of different colors and the mutations are highlighted with a black star.