Figure 3.

Pur attenuates Cd-induced apoptosis in rat cerebral cortical neurons. (A) Rats were treated with Cd (50 mg/L) and/or Pur (200 mg/kg) for 90 days. Apoptosis in the cerebral cortex was detected by a TUNEL assay. The TUNEL-positive cells were dark red (marked by black arrows). Scale bar: 50 μm. (B) Primary cortical neurons were pretreated with 100 μM Pur for 1 h, followed by 10 μM Cd exposure for 12 h. The morphology of the apoptotic cell nuclei was detected by DAPI staining (arrows point to apoptotic cells). Scale bars: 20 μm.