Figure 5.

Pur attenuates Cd-induced apoptosis via inhibiting the Fas/FasL-mediated mitochondrial apoptotic pathway in primary cortical neurons in vitro. Primary cortical neurons were pretreated with 100 μM Pur for 1 h, followed by 10 μM Cd exposure for 12 h. (A) The levels of Fas, FasL, FADD, and Cleaved Caspase-8; (B) ratio of tBID/BID; and (C) the levels of Cleaved Caspase-9, Cleaved Caspase-3, and Cleaved PARP1 were detected by Western blot. Left panel: representative Western blot image; right panel: quantitative analysis (mean ± SD; n = 3). The means labeled without a common letter (a, b, c) are significantly different, p < 0.05.