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Pur prevents Cd-induced AIF nuclear translocation in the rat cerebral cortex in vivo. Rats were treated with Cd (50 mg/L) and/or Pur (200 mg/kg) for 90 days, after which the cerebral cortices were subjected to AIF immunofluorescence staining. Upper panel: representative immunofluorescence staining images; lower panel: quantitative analysis (mean ± SD; n = 3). The means labeled without a common letter (a, b, c) are significantly different, p < 0.05. Scale. bar: 50 μm.
