Figure 3.

MEL alleviates oxidative damage in GC by inhibiting mitochondria. (a,b) GCs harvested from ovarian follicles were exposed to 200 μ M H₂O₂ for 1 h, and then treated with 10 μM MEL for 2 h. Mitochondria were stained with TMRM (red) and analyzed by FCM. (c,d) Mitochondrial membrane potential was monitored by a mitochondrial-specific dual fluorescence probe. GCs were treated with 200 μ M H₂O₂ for 1 h and incubated with 10 μM MEL for 2 h. Then, cells were incubated with JC-1 for 20 min and detected by FCM. (e,f) GCs treated with H₂O₂ for 1 h were then cultured with 10 μM MEL, and 0, 0.5,1 or 2 h later, JC-1 fluorescence was detected using FCM analysis. The +Y axis is 100 times the number of cells, and the +X axis is the intensity of cell fluorescence. Experiments were repeated in triplicate. Data represent mean ± S.E; n = 3. ** Represents p < 0.01 compared to control group.