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. 2021 Jul 8;11(7):229. doi: 10.3390/bios11070229

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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LFIA of RBD in artificially contaminated saliva. (a) Test strips with Au NPs; (b) Test strips after silver enhancement; (c) Test strips after galvanic-assisted Au deposition; (d) Test strips after gold enhancement: the numbers above the test strips show the concentration of RBD in the probe, in ng/mL, equal to 500 (1), 250 (2), 125 (3), 62.5 (4), 31.2 (5), 15.6 (6), 7.8 (7), 3.9 (8), 1.95 (9), 0.98 (10), 0.49 (11), 0.,24 (12), 0.12 (13), 0.06 (14), 0.03 (15). Concentration of RBD, in pg/mL, is equal to 15 (16), 7.5 (17), 3.8 (18), 1.9 (19), 0.9 (20), 0.05 (21), blank (0); (e) Dependences of the colorimetric signal in TZs versus RBD concentrations for various LFIA formats.

LFIA of RBD in artificially contaminated saliva. (a) Test strips with Au NPs; (b) Test strips after silver enhancement; (c) Test strips after galvanic-assisted Au deposition; (d) Test strips after gold enhancement: the numbers above the test strips show the concentration of RBD in the probe, in ng/mL, equal to 500 (1), 250 (2), 125 (3), 62.5 (4), 31.2 (5), 15.6 (6), 7.8 (7), 3.9 (8), 1.95 (9), 0.98 (10), 0.49 (11), 0.,24 (12), 0.12 (13), 0.06 (14), 0.03 (15). Concentration of RBD, in pg/mL, is equal to 15 (16), 7.5 (17), 3.8 (18), 1.9 (19), 0.9 (20), 0.05 (21), blank (0); (e) Dependences of the colorimetric signal in TZs versus RBD concentrations for various LFIA formats.