Table 1.

PCR primers and primer sequences used for the amplification of hsp40, hsp90 and β-actin gene transcripts.

Gene	Primer Name	Primer Sequence (5’-3’)	Size	Purposes
β-actin	RTB-actin_F	TTCACCATCGGCATTGAGAGGTTC	119 bp	Real-Time PCR
	RTB-actin_R	CACGTCGCACTTCATGATGGAGTT		Real-Time PCR
Mr-hsp40	F_GFP_hsp40	TGCAGGTTCGCATACAACAGTTGG	398 bp	RT-RCR
	R_GFP_hsp40	TTCGCCTGGAATAGCACTGATCAC		RT-RCR, Touchdown PCR in RACE
	RTF_GFP_hsp40	TCGCAAGCATACGAAGTTCTCAGC	136 bp	Real-Time PCR
	RTR_GFP_hsp40	CCGAAGAACATGTCGAAGATGTCC		Real-Time PCR
	Mr-HSP40T7_F	GGATCCTAATACGACTCACTATAGGGGGTGGAAAGAAGCCCCCAG		Gene silencing
	Mr-HSP40T7_R	GGATCCTAATACGACTCACTATAGGGCTCCTGGTCTCCCTCACCA		Gene silencing
Mr-hsp90	F_GFP_hsp90	CGAGTACCTGAACTTCCTCAATGG	565 bp	RT-RCR
	R_GFP_hsp90	CCTGTTCACGAGATTCACCAGTGA		RT-RCR, Touchdown PCR in RACE
	RTF_GFP_hsp90	GATGAGATGTGCTCCCTGAAGGAT	91 bp	Real-Time PCR
	RTR_GFP_hsp90	CCTGTTCACGAGATTCACCAGTGA		Real-Time PCR
	Mr-HSP90T7_F	GGATCCTAATACGACTCACTATAGGTGAGGCCTGACCATGGGGAA		Gene silencing
	Mr-HSP90T7_R	GGATCCTAATACGACTCACTATAGGGGCGACAAGTCTGTCCAGCA		Gene silencing
Green fluorescence protein (GFP)	GFP_F	TAATACGACTCACTAAGGGAGACACATGAAGCAGCACGACCT		Gene silencing
	GFP_R	TAATACGACTCACTATAGGGAGAAGTTCACCTTGATGCCGTTC		Gene silencing
UPM	LongUPM primer	CTAATACGACTCACTATAGGGCAAGCAGTGGTAACAACGCAGAGT		Touchdown PCR in RACE
	ShortUPM primer	CTAATACGACTCACTATAGGGC