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. 2021 Jun 23;11(7):932. doi: 10.3390/biom11070932

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow cytometry examination of hPDLFs after Annexin V/7AAD staining in relation to apoptotic cell death, with an exemplary representation of all events at 0–72 h in the control (C) and compressive force (CF) groups (a). The total percentage of Annexin V⁺/7AAD⁻ and Annexin V⁺/7AAD⁺ events in C showed a dead cell percentage of 15%, which decreased over time, while the proportion in the CF group significantly increased (b). Bars indicate the mean values ± standard deviation. A p-value < 0.05 was considered statistically significant. (°) Comparison of the CF with the respective control of the time point.