Figure 7.

SFI increased mitochondrial biogenesis in C2C12 mouse myoblasts cells through the AMPK and PI3K/Akt pathways: (a) protein expression of the AMPK signaling pathways; (b) protein expression of the Foxo3a, phosphate sites Ser 413, and Ser 253; (c) protein expression of the PI3K/Akt signaling pathways. The protein densitometry data were normalized with β-actin, and the p-AMPK-α, p-CREB, p-AKT, p-PI3K, p-Foxo3a Ser413, and Ser253 were normalized with the AMPK-α, CREB, AKT, PI3K, and Foxo3a protein contents.