Table 3.

Potency data for pairs of AIP analogs where the only sequence difference is a C-terminal phenylalanine or leucine.

agr group tested	Peptide containing Phe	EC50 [nM]a	IC50 [nM]b	Peptide containing Leu	EC50 [nM]a	IC50 [nM]b	Fold change
agr-I	AIP-I	170		I-tail, III-ring	1870		11.0
	AIP-II 9aa L9F		0.470	AIP-II 9aa		2.84	6.04
	Cmr2	71.8		Cmr2 F9L	1020		14.2
	Cmr3 L9F		2.15	Cmr3		75.8	35.2
agr-II	AIP-I		13.9	I-tail, III-ring		50.9	3.66
	AIP-II 9aa L9F	203		AIP-II 9aa	83.5		0.41
	Cmr3 L9F	210		Cmr3	>2000		---c
agr-III	AIP-I		2.13	I-tail, III-ring		31.8	14.9
	AIP-II 9aa L9F	>2000		AIP-II 9aa	>2000		---c
	Cmr1	0.929		AIP-III 9aa	28.9		31.1
	Cmr2	40.6		Cmr2 F9L	>2000		---c
	Cmr3 L9F		34.9	Cmr3	>2000		---c

^aCollected from agonism assay data or antagonism assay data obtained in S. epidermidis group-I–III reporter strains. See SI for methods.

^bCollected from agonism assay data or antagonism assay data obtained in S. epidermidis group-I–III reporter strains. See SI for methods.

^cNot calculated due to low potency.