Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 22;220(10):e202104093. doi: 10.1083/jcb.202104093

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Frazer et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S1. — Stronger TCR signal strengths increase CTL killing efficiency. (a) Killing assay with OTI CTLs incubated with EL4 pulsed with APL as shown at the CTL effector to target (E:T) ratios shown. Target lysis measured by LDH release, showing mean ± SD of triplicate values for one representative experiment of six. (b) LAMP-1 degranulation assay, showing LAMP-1 signal from OTI CTLs (gated on CD8) incubated with EL4 pulsed with APL (indicated by same colors as in [a]) or no EL4 (broken line) for 2.5 h. (c and d) Geometric mean fluorescence (c) and percentage LAMP1⁺ CTL against time (d) showing means of triplicate samples ± SD from one representative experiment of two.