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Increasing TCR signal strength increases CTL dwell time. Related to Fig. 1 a. OTI CTLs expressing Lifeact-mApple (green) and RFP-PACT (green) interacting with EL4 (blue), pulsed with N4, G4, or NP68 peptides. APL relates to the maximum-intensity projection representative time series of OTI CTLs encountering targets shown in Fig. 1. Scale bars = 5 µm; time min:s from start of recording. Playback speed, 10 frames per second.
