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Increasing TCR signal strength increases centrosome docking at the synapse. Related to Fig. 3 a. GzmB-TdTomato (red) OTI CTLs expressing Lifeact-EGFP (green) and BFP-PACT (white sphere) interacting with EL4 (blue), pulsed with N4, T4, or G4 peptides. APL relates to the representative time series maximum-intensity projection of OTI CTLs encountering targets shown in Fig. 3 a. Scale bars = 5 µm; time min:s from start of recording. Playback speed, 10 frames per second.
