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Duration of initial calcium fluxes are increased with higher-affinity ligands. Related to Fig. 6 a. OTI CTLs expressing GCAMP6m (green), Lifeact-mApple (red), and RFP-PACT (red sphere) interacting with EL4 (blue), pulsed with N4, T4, or G4. Representative maximum-intensity projection time series of OTI CTLs encountering APL pulsed targets. Scale bars = 5 µm; time min:s from start of recording. Playback speed, 10 frames per second.
