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. 2021 Jul 22;218(9):e20210580. doi: 10.1084/jem.20210580

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© 2021 Cavazzoni et al.

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Figure S4. — Analysis of V_H family usage and CDR-H3 length of Igh transcripts from B cells present in GCs after ZIKV NS1 or DENV NS1 immunization.(A) Expression of each V_H family as a percentage of total functional transcripts from GC B cells at each time point after immunization with ZIKV NS1 or DENV NS1. (B) CDR-H3 loop length variation at each time point after immunization with ZIKV NS1 or DENV NS1. (C) CDR-H3 loop length distribution of all sequences at all time points (upper panel) and comparison between clonal (clonotypes found more than once in the same LN) and single clones from mice immunized with ZIKV NS1 (lower panel). Dashed red line indicates the distribution of CDR-H3 length in FO B cells from WT BALB/c mice. GC B cells were sorted and sequenced from individual LNs and pooled for analyses (two to four mice per group from two independent experiments). Error bars represent SEM.