Table 2.
Metabolic and urine parameters of Wt and Flox/Pax8 + mice. Animals were induced with the low-dose doxycycline protocol (1 mg/mL). Water and food intake were monitored in metabolic cages under baseline conditions. 24 h urine and blood were collected for analysis. Statistical analysis was performed using Student’s t-test (n = 8 in both groups) *p < 0.05, **p < 0.01, ***p < 0.001
| Parameters | Wt/Pax8 + | Flox/Pax8 + |
|---|---|---|
| Body weight (g) | 25.2 ± 1.0 | 23.6 ± 1.8 * |
| H2O intake (g/g BW) | 0.19 ± 0.02 | 0.26 ± 0.01 * |
| Food intake (g/g BW) | 0.16 ± 0.02 | 0.19 ± 0.01 |
| Plasma | ||
| pH | 7.26 ± 0.03 | 7.27 ± 0.05 |
| Glucose (mg/dL) | 250.5 ± 8.9 | 238.9 ± 15.3 |
| Na+ (mM) | 144.7 ± 1.1 | 146.0 ± 1.1 |
| K+ (mM) | 3.9 ± 0.4 | 3.2 ± 0.1 * |
| Ionized Ca2+ (mM) | 0.96 ± 0.23 | 1.11 ± 0.05 |
| Cl− (mM) | 108.2 ± 1.5 | 109.2 ± 0.1 |
| Phosphate (mg/dL) | 5.9 ± 0.1 | 6.2 ± 0.3 |
| Prealbumin (mg/dL) | 1.76 ± 0.42 | 1.88 ± 0.15 |
| Creatinine (mg/dL) | 0.05 ± 0.01 | 0.07 ± 0.01 |
| BUN (mg/dL) | 40.6 ± 5.9 | 40.7 ± 3.3 |
| Urine | ||
| Urine (g/g BW) | 0.051 ± 0.009 | 0.083 ± 0.008 * |
| Creatinine (mg/dL) | 63.0 ± 4.2 | 42.8 ± 2.9 ** |
| Creatinine clearance (μL/min) | 109 ± 35 | 98 ± 23 |
| Na+ (mM)/Crea (mg/dL) | 1.7 ± 0.2 | 5.8 ± 1.1 ** |
| K+ (mM)/Crea (mg/dL) | 6.0 ± 0.3 | 7.1 ± 0.3* |
| Ca2+ (mM)/Crea (mg/dL) | 0.03 ± 0.01 | 0.01 ± 0.21* |
| Mg2+ (mg/dL)/Crea (mg/dL) | 1.65 ± 0.10 | 1.70 ± 6.25 |
| Phosphate (mg/dL)/Crea (mg/dL) | 6.7 ± 0.4 | 7.1 ± 0.6 |
| Cl− (mM)/Crea (mg/dL) | 4.0 ± 0.2 | 5.0 ± 0.3 |