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. 2021 Jul 23;12:4482. doi: 10.1038/s41467-021-24825-y

Fig. 8. HDAC6 activates native Panx1 channels in T lymphocytes.

Fig. 8

a Exemplar recording from a freshly isolated mouse CD4+ T cell that responded to PE (20 μM) with an increase in whole-cell CBX-sensitive current and I–V characteristics of Panx1. b Summary data (mean ± s.e.m, blue lines) showing CBX-sensitive whole-cell current density in mouse CD4 + naïve T cells, before and after bath application of 20 μM PE (n = 8; 5 Treg and 3 Teff cells examined over 3 independent experiments); one-tailed paired t-test (t = 2.014, df = 7). c Representative whole-cell recording from Jurkat T cell that showed increased CBX-sensitive PANX1-like current in response to PE (20 μM). d Summary data (mean ± s.e.m, blue lines) showing CBX-sensitive currents before and after PE stimulation on individual Jurkat T cells; one-tailed paired t-test (n = 13 cells examined over 3 independent experiments; t = 3.682, df = 12). e, f Inside-out recording from a Jurkat T cell showing activation by HDAC6 of a PANX1-like channel (e), with corresponding unitary I–V (f). g Schematic illustrates the RhoA-mDia-HDAC6 signaling pathway interposed between the Gαq-coupled α1-AR and Panx1 channel activation by lysine deacetylation and alternative pathway(s) not yet defined. PE phenylephrine, NE norepinephrine, CBX carbenoxolone.