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. 2021 Jul 23;12:4511. doi: 10.1038/s41467-021-24721-5

Fig. 4. Lipopolysaccharide protects S. flexneri from septin cage entrapment.

Fig. 4

a Representative Airyscan confocal images showing the binding of septins in vitro to S. flexneri (WT, top) or ΔrfaC (O-antigen–, bottom). Scale bar, 5 μm (inset, 1 μm). b Percentage of bacteria recruiting septins in vitro. Quantifications represent mean ± SEM from n = 895 (WT), n = 689 (ΔrfaC) S. flexneri cells distributed in 3 independent experiments. *p = 0.0249 by two-tailed Student’s t-test. c Airyscan confocal images of HeLa cells infected for 3 h 40 min with S. flexneri str. afaE (WT, top) or S. flexneri ΔrfaC (bottom) and stained for SEPT7 and Hoechst. SEPT7 cages are indicated with arrowheads. S. flexneri afaE strain (hyperinvasive) was used to compare WT infection to S. flexneri ΔrfaC. Scale bar, 5 μm (inset, 2 μm). d Percentage of septin caged bacteria in HeLa cells infected for 3 h 40 min. Data represent the mean ± SEM from n = 1695 (afaE) and n = 1565 (ΔrfaC) S. flexneri cells distributed in 8 independent experiments. **p = 0.0033 by two-tailed Student’s t-test.