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. 2021 Jul 23;11:15090. doi: 10.1038/s41598-021-93909-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Zebrafish kl mutants become thinner and undergo premature death. (a–d) The kl^+/+ female (a), kl^+/+ male (b), kl^−/− female (c) and kl^−/− male (d) zebrafish at 4 mpf. The kl^−/− fish showed no morphological defects at this stage. (e–l) Lateral and dorsal views of head in kl^+/+ female (e,i), kl^+/+ male (f,j), kl^−/− female (g,k) and kl^−/− male (h,l) at 5 mpf. Note that the eyeball is protruding to the outside in the kl^−/− female. (m–p) The kl^+/+ female (m), kl^+/+ male (n), kl^−/− female (o) and kl^−/− male (p) zebrafish at 6 mpf. The kl^−/− fish became skinny. (q) The kl^+/+ fish (n = 32) survived over 9 mpf. All of the kl^−/− fish (n = 28) underwent premature death before 9 mpf.