Figure 1.
KC (LSL:KrasG12D:Pdx1-CRE) mouse model. (a) Schematic representation of the genetic construct for conditional KrasG12D expression under the control of Pdx1 promoter (CRE-Lox system). Primers used for KrasG12D conditional PCR are noticed. (b) Top: KrasG12D conditional PCR showing LSL cassette recombination in pancreas, but not tail, from KC mouse, and not in WT mouse. Bottom: KrasG12D genotyping PCR showing heterozygosity in KC mice (WT and KrasG12D alleles) and homozygosity in WT mouse (WT allele). Uncropped gels are provided in Supplementary Figure S2.