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. 2021 Jul 23;12:4485. doi: 10.1038/s41467-021-24515-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Circos⁵⁸ graph displaying the coverage and SNV-rich regions (single-nucleotide errors) of the strain-oblivious assemblies (Flye and Canu) and their strain-separated counterparts obtained with Strainberry (ssFlye and ssCanu, respectively) compared to the reference sequences of the two E. coli strains present. The external graduated scales reflect the genomic positions of the corresponding reference genomes. b Average nucleotide identity (ANI) and c duplication ratio of assemblies.