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. 2021 Jul 20;135(14):1689–1706. doi: 10.1042/CS20210198

Figure 5. Effect of GUDCA in stabilizing calcium homeostasis in vitro.

Figure 5

(A) Comparisons of fluorescence intensity in response to PA between vehicle and GUDCA-pretreated cells. The cells were analyzed using Fluo-3/Fura-Red dual-labeling confocal microscopy; scale bars: 50 µm (n = 3/group). (B) Relative Fluo-3/FruaRed ratio and (C) the number of cells with significant changes in fluorescence. (D) Quantitative real-time PCR and (E) Immunoblot measurement of SERCA2 in the HepG2 with indicated interventions (n = 4/group). All data are presented as the mean ± SEM and analyzed by two-tailed unpaired Student’s t test for (A), one-way ANOVA followed by the Bonferroni post hoc test for (B) and (C); *P<0.05, **P<0.01, ***P<0.001 versus control.