Table 4.

List of nucleic acid-based diagnostic tools developed on the basis of emergency use authorized by FDA [79].

Source	Mechanism of Detection	Site/antibody Target	Time limit	Detection ​limit	Point of care
Thermo Fisher	qReverse transcriptase -PCR	Open reading ​frame1ab, ​Nucleoprtein coding gene, ​Surface protein coding-gene	>2h	10 copies/microliter	no
Roche	qReverse transcriptase -PCR	Open reading ​frame ​1a	>2h	9 copies/microliter	no
PerkinElmer	qReverse transcriptase -PCR	Open reading ​frame ​1 ​ab, ​Nucleoprotein coding gene	>2h	0.025 copies/microliter	no
Sherlock Bioscience	CRISPR ​+ ​LFA	Surface protein-coding ​gene, Open reading frame1ab	60 ​min	10-100 copies/microliter	yes
Cepheid	qReverse transcriptase -PCR	Nucleoprotein coding gene, Envelope protein coding-gene	45 ​min	0.25 copies/microliter	yes
BioFire	qReverse transcriptase -PCR	Open reading ​frame 8, ​Open reading ​frame ​1 ​ab	50 ​min	0.33 copies/microliter	yes
Mesa Biotech	Reverse transcriptase -PCR ​+ ​LFA	Nucleoprotein coding gene	30 ​min	200 copies/reaction	yes
Abbott	Isothermal nucleic acid amplification	RNA dependent RNA polymerase-gene	5/13 ​min	0.125 copies/microliter	yes
CDC	qReverse transcriptase -PCR	Nucleoprotein coding gene	>2h	3.2 copies/microliter	no
Mammoth Bioscience	CRISPR ​+ ​LFA	Envelope protein-coding gene, Nucleoprtein gene	30 ​min	70-300 copies/microliter	yes