Table 2.
Details of primers used.
| Gene | Accession Number | Primers | Length (bp) |
|---|---|---|---|
| Nox4 | NM_016931.3 | 5′–TGAACTATGAGGTCAGCCTCTG–3′ 5′–TCTCACGAATCTCCTCATGGT–3′ |
107 |
| GPx1 | NM_201397 | 5′–CAGTCGGTGTATGCCTTCTCG–3′ 5′–GAGGGACGCCACATTCTCG–3′ |
105 |
| GPx3 | NM_002084 | 5′–GAGCTTGCACCATTCGGTCT–3′ 5′–GGGTAGGAAGGATCTCTGAGTTC–3′ |
94 |
| GPx4 | NM_001039847 | 5′–GAGGCAAGACCGAAGTAAACTAC–3′ 5′–CCGAACTGGTTACACGGGAA–3′ |
100 |
| P16INK4a | NM_000077.4 | 5′–CTTCGGCTGACTGGCTGG–3′ 5′–TCATCATGACCTGGATCGGC–3′ |
129 |
| P21 | NM_078467 | 5′–TGTCCGTCAGAACCCATGC–3′ 5′–AAAGTCGAAGTTCCATCGCTC–3′ |
139 |
| IL–6 | NM_000600 | 5′–ACTCACCTCTTCAGAACGAATTG–3′ 5′–CCATCTTTGGAAGGTTCAGGTTG–3′ |
149 |
| PlGF | NM_001207012 | 5′–GAACGGCTCGTCAGAGGTG–3′ 5′–ACAGTGCAGATTCTCATCGCC–3′ |
187 |
| sFlt–1 | NM_001159920 | 5′–TTTGCCTGAAATGGTGAGTAAGG–3′ 5′–TGGTTTGCTTGAGCTGTGTTC–3′ |
117 |
| B2M | NM_004048.2 | 5′–CTTATGCACGCTTAACTATCTTAACAA–3′ 5′–TAGGAGGGCTGGCAACTTAG–3′ |
127 |
| YWHAZ | NM_001135702.1 | 5′–ACTTTTGGTACATTGTGGCTTCAA–3′ 5′–CCGCCAGGACAAACCAGTAT–3′ |
94 |
| GAPDH | NM_002046.3 | 5′–GGAAGCTTGTCATCAATGGAA–3′ 5′–TGGACTCCACGACGTACTCA–3′ |
102 |