Table 3.

BRAF concordance analysis.

Patient No		Tumor			Liquid Biopsy 1		Liquid Biopsy 2
	P/M 1	qPCR	NGS	VAF 2 %	NGS	VAF %	NGS	VAF %
1	M	V600	V600E	48.6	V600E	7.0	V600E	93.0
2	M	V600	V600E	18.7	V600E	25.0	V600E	35.0
3	M	V600	V600E	37.5	V600E	2.5	V600E	5.2
4	M	WT 3	WT	0.0	WT	0.0	WT	0.0
5	M	V600	NA 4	-	V600E	35.0	WT	0.0
6	P	V600	V600E	43.9	V600E	43.2	NA
7	M	V600	V600E	31.4	V600E	0.7
8	P	WT	WT	0.0	WT	0.0
9	P	V600	WT *	0.0	V600E	0.3
10	P	V600	V600E	50.7	V600E	16.8
11	P	WT	WT	0.0	WT	0.0
12	P	V600	V600R	33.0	V600R	0.2
13	M	V600	NA	-	V600E	6.4
14	P	V600	NA	-	WT *	0.0

¹ P/M—primary tumor/metastasis; ² VAF—variant allele frequency; ³ WT—wild type; ⁴ NA—not analyzed; discordant results are marked with an asterisk.