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. 2021 Jun 29;10(7):1626. doi: 10.3390/cells10071626

Figure 5.

Figure 5

Lipid-lowering drugs affect HCV infection of lipoprotein receptor-deficient cell lines. Drug-induced cell cytotoxicity of lipoprotein receptor-deficient cell lines (A). Huh-7.5 derived SR-B1 KO #3 (light orange bars). LDLr KO #1 (grey bars) and DKO #1 (light blue bars) were treated with 1 µM simvastatin, 5 µM fenofibrate or 1 µM alirocumab for 96 h. Cell viability was determined by MTT Assay. (B) HCVcc infection of KO cells (B). SR-B1 KO #3, LDLr KO #1, and DKO #1 were treated with indicated drugs 24 h before infection with an FLuc reporter GT2a HCV virus (Fluc-Jc1) in the presence of the compounds or solvent control. 4 h later, virus was removed and drugs applied for extra 72 h before cell lysis and FLuc measurement. FLuc activities were normalized to DMSO control. Graphs represent 3 independent experiments performed in triplicates. Bars show the average and error bars in SD. Black dots plot individual values. Multiple comparison two-way ANOVA with a post hoc Dunnett correction was performed for statistical analysis (α = 0.05, DF = 40). * p ≤ 0.05; ** p ≤ 0.01; **** p ≤ 0.0001.