Skip to main content
. 2021 Jul 11;10(7):1756. doi: 10.3390/cells10071756

Figure 4.

Figure 4

Cellular uptake of IAV DI vRNAs and DExD/H-box helicase 58 (DDX58) expression. SARS-CoV-2-infected Calu-3 cells (MOI = 0.03) were treated with IAV DIPs (DI244 or OP7) at 1 hpi. For DI244 and OP7 infection, 10% (v/v) (100 µL culture volume) of highly concentrated cell culture-derived DIP material was used [29,30]. At indicated times post-infection, cells were lysed to allow for total RNA extraction, required for (A) quantification of intracellular DI vRNAs and (B) analysis of DDX58 gene expression. Illustration includes data from one experiment. (A) Intracellular DI vRNA levels during SARS-CoV-2 and IAV DIP co-infection. Cells were assayed for viral RNAs by real-time RT-qPCR. (B) DDX58 gene expression analysis. DDX58 (encoding for RIG-I) transcript levels were quantified by real-time RT-qPCR and expressed as fold change (relative to untreated, uninfected cells).