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Effect of PDA/GO on MAPKs signaling pathways. The cells were cultured on the PDA/GO substrate for 7 days and (A) Phosphorylation of ERK1/2 (42–44 kDa), p38 (38 kDa), and JNK (46–54 kDa) was assessed. (B) Cells were transfected with integrin α5/β1-specific siRNA, and the protein levels of integrin α5, β1, and the ERK1/2, p38, and JNK phosphorylation levels were examined after 4 days of osteogenic induction.
